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| Registros recuperados: 35 | |
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| Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G. |
| Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012 |
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| Bumunang,Emmanuel Wihkochombom; Babalola,Olubukola Oluranti. |
| This study was done to examine the rhizobacteria from field grown Genetically Modified (GM) maize and its non-GM counterpart. Rhizospheric soil samples were collected at 30 days after sowing (DAS) and at post-harvest from two experimental fields in Gauteng, South Africa. Total rhizobacteria (cfu/g) in GM and non-GM soil samples was not significantly different across the different media 30 DAS and at post-harvest. Rhizobacterial isolates obtained were biochemically characterized using the analytical profile index. Species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Stenotrophomonas, Achromobacter, Ewingella and Bacillus were screened in vitro for plant growth promoting traits such as, ammonia production, catalase activity, indole acetic acid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetically modified maize; Plant growth promoting traits; Rhizosphere; Rhizobacteria. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100001 |
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| Tahir,Muhammad; Khalid,Umaira; Ijaz,Muhammad; Shah,Ghulam Mustafa; Naeem,Muhammad Asif; Shahid,Muhammad; Mahmood,Khalid; Ahmad,Naveed; Kareem,Fazal. |
| Abstract This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150–100 NP kg ha−1) and half dose (75–50 NP kg ha−1) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments. The cultivar Punjab-2011 produced the higher chlorophyll contents, crop growth rate, and the straw yield at half dose of NP fertilizer; while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phosphorous; Phosphorous solubilizing bacteria; Bioavailability; Rhizosphere; Low fertilizer input. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500015 |
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| Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G. |
| Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metagenomics; Rhizosphere; Wheat. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018 |
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| Brasil,Carlos; Matsumoto,Leopoldo Sussumu; Nogueira,Marco Antonio; Spago,Flavia Regina; Rampazo,Luís Gustavo; Cruz,Marcio Ferreira; Andrade,Galdino. |
| The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500022 |
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| Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. |
| A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S... |
| Tipo: Journal article |
Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018 |
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| Souchie,Edson L.; Azcón,Rosario; Barea,Jose M.; Silva,Eliane M.R.; Saggin-Júnior,Orivaldo J.. |
| This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araxá apatite; Phosphate solubilization; Co-inoculation; Plant growth promotion; Rhizosphere. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300023 |
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| Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir. |
| A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013 |
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| Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad. |
| In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031 |
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| Fortes Neto,Paulo; Fortes,Nara Lúcia Perondi; Silva,Eliana Maria de Araújo Mariano da; Brambatti,Fabiana. |
| ABSTRACT Studies on the survival of pathogenic microorganisms in the soil after use of wastewater for fertilization of agricultural crops report the effects of moisture, pH, organic matter, and soil temperature on microorganisms. There are few studies that assess the survival of these microorganisms in the rhizosphere of plants fertilized with wastewater. Thus, the aim of this study was to quantify the number of fecal coliforms and rhizobacteria (fluorescent Pseudomonas spp., Bacillus spp,) in the rhizosphere of winter and summer crops fertilized with wastewater. In the experiment, we used 20 plots, and each plot occupied an area of 200 m2. The treatments used in the winter crop consisted of uncultivated plots and single crops of wheat, triticale, black... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizosphere; Reclaimed water; Wastewater. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100414 |
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| Registros recuperados: 35 | |
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